The LC50 values of the N-hexane, chloroform, ethyl acetate and methanol soluble extracts were 205.984 μg/ml, 34.493 μg/ml, 66.235 μg/ml and 751.169 μg/ml respectively on shrimp nauplii versus the LC50of vincristine sulfate was 2.254 μg/ml. Total antioxidant capacity of different extracts of the plant were calculated using the equation (y = 0.0077x + 0.176, R2 = 0.9954) obtained from reference standard ascorbic acid and expressed as mg/gm equivalent of ascorbic acid (AAE) (Table 2). It has been assumed that approximately 250 species of medicinal plants are employed for the preparation of traditional medicines which is the 50 % of total species of plants grown in Bangladesh [5]. Membrane Stabilization method is a method that is usually employed to evaluate in-vitro anti-inflammatory activity of any possible drug substances. Pinnae 6-8 pairs; basal pair largest, ovate-deltoid or ovate-lanceolate, 3-9 × 2.5-4.5 cm, stalked (stalks 0.3-1 cm), apex acuminate; proximal pinnae inequilateral, basal basiscopic pinnules largest. 2014;59(3):231–9. The capacity of chloroform and ethyl acetate fractions are also noteworthy (98.24 μg/ml and 77.99 μg/ml) and N-hexane showed minimal (334.76 μg/ml) activity to DPPH as increased IC50 value decreases inhibitory activity. Cheilanthes tenuifolia, a member of the Pteridaceae family, is an evergreen and small fern that can grow up to 70 cm in height. Cure eczema and stomachache. CAS  J Agric Food Chem. Sarah QS, Anny FC, Misbahuddin M. Brine shrimp lethality assay. 24. The villagers were mainly willing to share their information of herbal medication. 3). California Privacy Statement, Cite this article. To determine the cytotoxicity of plant extract, brine shrimp lethality assay is an important parameter by observing the capability of extract to kill a laboratory cultured larvae (nauplii). Benniamin A. Cheilanthes tenuifolia (Burm.f.) It may be assumed that these extracts can be considered as good source of thrombolytic and membrane stabilizing agents due to presence of high number of flavonoids. Fil. Then the collected RBC was kept in a sterile test tube with an anticoagulant EDTA. Root. Enough solvent was added to ensure that all the silica gel was properly solvated [22]. Plants, their whole parts or derivatives, most important natural resource are used for therapeutic benefits. Noun 1. Suitable pH: acid, neutral and basic (alkaline) soils. They have been selected to provide a mix of different plant sizes and growing conditions. Hussain F, Islam MA, Bulbul L, Moghal MR, Hossain MS. et al. 22. 1. Manage cookies/Do not sell my data we use in the preference centre. Int J Pharm Sci Res. f.) Sw. Anc Sci Life. International Journal of Phytomedicine and Phytotherapy, Clinical Phytoscience In vitro thrombolytic potential of root extracts of four medicinal plants available in Bangladesh. Define Cheilanthes distans, Cheilanthes tenuifolia. Cheilanthes tenuifolia (N. L. Burman) Swartz, Syn. Separation techniques: chromatography. Dhar KS, Wahed TB, Hasan AN, Wahed SB. Screening of 11 ferns for cytotoxic and antitumor potential with special reference to Pityrogramma calomelanosPityrogramma calomelanos In the present study, different crude methanolic extract fractions of Cheilanthes tenuifolia were evaluated for their possible thrombolytic activities. J Appl Pharm Sci. Cheilanthes tenuifolia (Burm. of the supernatant was measured at 540 nm. Indian J Tradit Know. 100 μl solutions of different fractionates along with crude extracts and pure compounds were added separately to each Eppendorf tube containing pre-weighed clot. 2017;12(2):5–9. Treatment of lumbago . The other pair was maintained at 0-5 °C in an ice bath. Cheilanthes tenuifolia (Burm.f.) Summary Nomenclature Taxon Concepts Subordinates Vernacular Applications Feedback. ... Cheilanthes farinosa Kaulf. After 30 min, the absorbance was measured at 517 nm. Measuring phenolics in the winery. In our study, methanol fraction of Cheilanthes tenuifolia showed highest amount of total phenol content (11.32 ± 0.28 mg/gm GAE) followed by chloroform fraction (9.71 ± 0.31 mg/gm GAE) > N-hexane fraction (6.69 ± 0.67 mg/gm GAE) > ethyl acetate fraction (5.36 ± 0.54 mg/gm GAE), (Table 2). This hardy fern grows in dry, well-drained areas, often in full sun. Int J Pharm Sci Res. Chaturvedula VP, Prakash I. Cheilanthes austrotenuifolia: is known as the Rock Fern. Besides Nayar 2, and Kaushik & Dhiman 3 also reflected lights of medicinal uses of some pteridphytes of India. Brand-Williams W, Cuvelier ME, Berst C. Use of free radical method to evaluate antioxidant activity. Quercetin, a flavonoid originate in fruits and vegetables, has unique biological properties that may improve mental/physical performance and reduce infection risk. 2016;3(2):156–60. In the case of Dicranopteris linearis, the rhizome is used as anthelmintic in Assam while the fronds are used for asthma in Madagascar (Manickam and Irudayaraj, 1992). Files are available under licenses specified on their description page. The n-hexane soluble fraction of the plant extract was loaded in the top of the column and eluted in test tubes with different solvent system composed of n-hexane, chloroform and ethyl acetate (from non polar to polar) in different proportions (200 ml). There are many methods to decide in-vitro antioxidant activities and DPPH scavenging activity is one of them. On the other hand sterile distilled water, a negative control exhibited a negligible percentage of lysis of clot 8.511%, ethyl acetate extract 31.594%, N-hexane fraction 12.100%, methanol fraction 17.006% and chloroform fraction 41.261% showed lysis of clot respectively. Google ScholarÂ. 2014;21(1):71–6. A buffer was prepared using monosodium phosphate and its conjugate base, disodium phosphate having pH 7. Chloroform fraction was found most potent thrombolytic potential compared to standard SK. 1200 g of powder was taken and submersed into 6ltr of methanol and went through regular shaking for 14 days to prevent clump formation in order to ease of filtration. After the mixture had cooled to room temperature, the absorbance of the solution was measured at 695 nm against blank. Preliminary phytochemical analysis was performed for all the fractions showed the presence of alkaloids, glycosides, tannins, and steroids which are listed in Table 1. The absorbances of the solutions were measured at 765 nm using a spectrophotometer against blank. At first, 5 g of crude extract was taken in a 500 mL beaker. Sources of data Cheilanthes tenuifolia (inventories, collectors / déterminateurs, organizations, data transmission) In continuing your browsing of this site, you accept the use of cookies to offer you suitable content and services and realize visits statistics. Indian J Pharmacol. Khatun M, Rahman M, Haque T, Rahman MM, Akter M, Akter S, Jhumur A. Cytotoxicity potentials of eleven Bangladeshi medicinal plants. Top Level Regions; Hawaii: FAC Hawaii; South Pacific Islands: FAC Related Links. Senguttuvan J, Paulsamy S, Karthika K. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. J Ethnobiol Ethnomed. 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