Primer pairs were designed to amplify partial DNA fragments of cytochrome b … It encodes the β‐chain of flavocytochrome b 245 (also called as gp91 phox or NOX2).  |  2018 Aug 17;3(2):925-932. doi: 10.1080/23802359.2018.1501284. Animals of the same species, both the endangered animal species and domestic animals, were clustered together in the neighbor-joining tree. A novel strategy for avian species identification by cytochrome b gene. and Liao, S.P. Kumar VP, Shukla M, Rajpoot A, Thakur M, Nigam P, Kumar D, Mehta AK, Goyal SP. NLM The species is the primary unit of concern in biodiversity, conservation, and other biological issues. Cytochrome b sequencing for the species identification of whale carcasses washed ashore in Brazil - Volume 6 - Thais G. Corrêa Sholl, Jailson Fulgencio de Moura, Paulo Henrique Ott, Cibele R. Bonvicino, Estéfane C. Reis, Davi Castro Tavares, Salvatore Siciliano This requires the identification of the species before individual analysis can take place. Copyright © 2001 Elsevier Science Ireland Ltd. All rights reserved. Identification of endangered petrel species from poor quality feather debris using cytochrome b sequences. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. The PCR-RFLP-method is an inexpensive tool in forensic science, even if the template is degraded or contains only traces of DNA from various species. Mitochondrial DNA is very useful for its multiple presences in cells. SPInDel Analysis of the Non-Coding Regions of cpDNA as a More Useful Tool for the Identification of Rye (Poaceae: A novel droplet digital PCR human mtDNA assay for fecal source tracking. hopeful technique for meat species identification. The amplification of the 402 bp fragment of the cytochrome b gene was found to be able to detect rhinoceros DNA even in the ratio of 1:19 with Holstein cow DNA. We use cookies to help provide and enhance our service and tailor content and ads. A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. Gonçalves R, Parik J, Freitas AI, Jesus J, Villems R, Brehm A. Forensic Sci Int. We sequenced a 396-bp region of the mitochondrial cytochrome b gene of the most common clinically important Candida species: Candida albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. krusei, … USA.gov. Two strategies for extracting DNA from … Species Identification of Kachuga tecta Using the Cytochrome b Gene. Ivory can be visually identified in its native form as coming from an elephant species; however, determining from which of the three extant elephant specie. Identification of Different Species of Mammalians Involved in Zoonoses as Reservoirs or Hosts by Sequencing of the Mitochondrial DNA Cytochrome B Gene . mammals, birds, reptiles, amphibians and fishes). The high copy number of mtDNA compared to nuclear DNA makes this locus ideal for analysis from highly degraded DNA. Mitochondrial DNA B Resour. Three unknown samples of animal remains were identified by this system. Even if sequences for many species are … Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. Cytochrome b/b6 non-covalently binds two heme groups, known as b562 and b566. The most of animal mtDNA is coding 37 genes [1]. A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. Because adulteration of the drug with substances from other animals such as horses, cattle, and pigs has been found, we examined PCR methods based on the sequence of the cytochrome b gene for source species identification. Hsieh HM, Tsai CC, Chiang HL,Tsai LC, Huang NE, Shih RTP, Linacre A, Lee JCI. We developed a species identification based on sequencing of the cytochrome b (cyt b) gene directly from the DNA extracted from the clinical specimen. These shells may come from endangered and protected species, requiring the identification of the species present to enforce national and international legislation. The sequences were analyzed using the Sequence Analysis/Seqscape v2.1 software (Applied Biosystems). Species identification by cytochrome b gene: Casework samples mammals, birds, reptiles, amphibians and fishes). The inconsis- We report on the design of five c … Species identification using the cytochrome b gene of commercial turtle shells Forensic Sci Int Genet. NCI CPTC Antibody Characterization Program. Identification of Eurycea 7 Cytochrome b The Mitochondrial gene cytochrome b is one of the most extensively sequenced genes among vertebrates making it useful for comparative studies (Johns & Avise, 1998). The sequences were analyzed using the Sequence Analysis/Seqscape v2.1 software (Applied Biosystems). developed for the identification of nucleotide polymorphisms in the Cyt b gene capable of differentiating biological samples derived from the paca from those of domestic species commonly used for the production of meat in the Neotropics (cow, goat, sheep, and pig). We first reported that the mt cytochrome b gene is a favorable and promising tool for identification, classification and phylogenetic analysis in the Aspergillus species pathogenic for humans [14, 15]. Consequently, it was found that the PCR‐RFLP method was sufficient for rapidly screening cod products, and in cases that require conclusive identification, phylogenetic … Lopez-Oceja A, Gamarra D, Borragan S, Jiménez-Moreno S, de Pancorbo MM. Diagnostic Cytochrome b gene profiles for the identification of paca (Cuniculus paca) bushmeat: ... based on single nucleotide polymorphisms of the cytochrome b gene, that can be used to differentiate biological material derived from paca from those of domestic species commonly used as sources of meat. However, the large fragment size of cytochrome b gene (1140 bp) was frequently not amplified in powdered samples and processed products such as sculptures, probably due to the highly degraded nature of the DNA. mammals, birds, reptiles, amphibians and fishes). Mitochondrial DNA B Resour. The cytochrome b gene of mitochondrial DNA (mtDNA) has been used in phylogenetic studies , and species identification . Lee JC(1), Tsai LC, Liao SP, Linacre A, Hsieh HM. Evolution of the This thesis discusses the mitochondrial gene cytochrome b and its utility in population genetics and identification of larval amphibians. A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. The sequences derived were used to … All sequences were submitted to the GenBank including new species which were not observed in the databases. The cytochrome b gene on the mitochondria has been used successfully in spe- cies identification [9, 10, 11] and in taxonomic and phy- logenetic studies [12, 13, 14]. The results of sequence comparison showed that the percentage range of sequence diversity in the same species was from 0.25 to 2.74%, and that between the different species was from 5.97 to 34.83%. COVID-19 is an emerging, rapidly evolving situation. Species identification by cytochrome b gene: Casework samples L. Caine´ a,*, G. Lima a, L. Pontes a, D. Abrantes a, M. Pereira a, M.F. HHS However, the complete sequencing of the cyotchrome b gene is time-consum- ing and laborious due to its size (1140 bp). Lima NGDS, do Carmo AO, de Souza RCC, Kalapothakis E, Eterovick PC. 7-18. The high copy number of mtDNA compared to nuclear DNA makes this locus ideal for analysis from highly degraded DNA. The sequences derived were used to identify the biological origin of the samples by aligning to cytb gene sequence entries in nucleotide databases using the program BLAST. Water Res. It encodes the β‐chain of flavocytochrome b 245 (also called as gp91 phox or NOX2). Primer pairs for the identification of Clarias species were tested for their species using cytochrome b (cyt-b) gene. Species identification for forensic purposes is being increasingly used, as the value of non-human evidence is realized. Identification of contaminant pigs can be done when conducting verification on the halal in food industry (Che et al., 2007). Direct estimates of mitochondrial cytochrome b gene … Forensic Sci Int 2001; 122: 7-18. One of them is gene for cytochrome b (CytB). 2016 Jul;23:159-165. doi: 10.1016/j.fsigen.2016.05.001. Background: Species identification is an important process to identify the origin of meat, adulteration and for cooked and processed meat. The partial sequence (402 bp) of cytochrome b gene has been proved to be of value in the identification of animal species from unknown samples. In the initial identification of species from unknown powdered material, all the … The recently described species of Candida , C. dubliniensis , associated with mucosal candidiasis in human immunodeficiency virus-infected individuals, … Hsieh HM, Huang LH, Tsai LC, Liu CL, Kuo YC, Hsiao CT, Linacre A, Lee JCI. The partial sequence of cyt b gene from these specimens were amplified and sequenced. Heteroplasmy was detected in the formosan macaque, domestic pig and domestic cats. 2. The test on the shell of the animal was used to identify samples from the species Kachuga tecta. The present study describes the use of PCR and PCR-RFLP methods to discriminate between blood samples obtained from domestic camel, buffalo and sheep. Asini Corii Collas (ACC; donkey glue) is a crude drug used to promote hematopoiesis and arrest bleeding. Epub 2016 May 6. Abstract. J Forensic Sci 2006; 51: 52-6. In plants and cyanobacteria, cytochrome b6 consists of two subunits encoded by the petB and petD genes. 2 Materials and methods 2.1 Strains Would you like email updates of new search results? 2. Recent estimates of the number of species range from 10 to 100 million (Wilson 1998). It is a necessary component of the NADPH oxidase complex in phagocytes, such as granulocytes, monocytes, and macrophages. Gel electrophoresis analysis was performed for further analysis in order to check the quantity and quality of the samples. 3. The PCR-RFLP-method is an inexpensive tool in forensic science, even if the template is degraded or contains only traces of DNA from various species. The gene is 1,140 basepairs long in most vertebrates and shows little intraspecies variation, but sufficient interspecies variation. 2009 Mar;3(2):67-73. doi: 10.1016/j.fsigen.2008.10.005. Skuza L, Filip E, Szućko I, Bocianowski J. Int J Mol Sci. A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. 2018 Feb 20;3(1):250-255. doi: 10.1080/23802359.2018.1438858. Length of CytB gene is 1140 bp and has some Validation of cytochrome b sequence analysis as a method of species identification. In this report, the species of one complete ventral turtle shell and 33 fragmented turtle shells provided by the Council of Agriculture (COA) dating from 2002 were identified. This requires the identification of the species before individual analysis can take place.  |  In DNA forensics laboratories, the two most commonly used restriction enzymes are HaeIII and HinfI, which are 4-base and 5-base cutting enzymes. Species identification for forensic purposes is being increasingly used, as the value of non-human evidence is realized. These primers were mixed in the ratio of 1: 0.2: 3:3: 0.6: 3: 0.6: 2 for SIM: G: Ch: C: B: S: P: H and used together for the multiplex PCR. Material and Methods Eight polymorphic partial cytochrome b sequences ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. This method allowed the identification of fish species in all samples. The applicability of this method to the forensic field is demonstrated by simulated casework conditions where different types of samples including problematic specimens such as hair, bone samples, bristles and feathers were investigated to identify the species. Species Identification and PCR‐RFLP Analysis of Cytochrome b Gene in Cod Fish (Order Gadiformes) Products Tetsuya Akasaki Authors Akasaki, Tomonaga, and Sato are with Central Customs Laboratory, Ministry of Finance, 6‐3‐5 Kashiwanoha, Kashiwashi, … The partial sequence of cytochrome b gene adopted in this study proved to be usable for animal identification. A total of 100 tortoise shell specimens collected from the National Council of Agriculture (COA), Taiwan, were used in this study. Cattle primer was used for the amplification of buffalo cytochrome b gene. Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. 2008 Jan 15;174(1):87-8. doi: 10.1016/j.forsciint.2006.10.024. Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. One of them is gene for cytochrome b (CytB).  |  This site needs JavaScript to work properly. Copyright © 2021 Elsevier B.V. or its licensors or contributors. Forensic Sci Int Genet. New cyt b gene universal primer set for forensic analysis. Cyt b gene is a gene that is often used to compare multiple phylogenetic species in the same genus or family, the diversity of the cyt b gene has been used to detect the source of milk derived from cattle (Bos), Pinheiro a,b b a Instituto Nacional de Medicina Legal, Delegac¸a˜o do Porto, Portugal Faculdade de Cieˆncias da Sau´de, Universidade Fernando Pessoa, Portugal Abstract. Zhu K, Suttner B, Pickering A, Konstantinidis KT, Brown J. Forensic Sci Int 2003; 136: 1-11. The present study was conducted to identify cattle (Bos taurus) and buffalo (Bubalus bubalis) by using mitochondrial cytochrome-b (Cyt-b) gene.Size of the gene is 1140 bp, but we amplified 359 bp that is cleaved by specific … To study the identification and phylogeny of pathogenic isolates of Aspergillus, we designed primers from known cytochrome b amino acid sequences. A DNA technique has been established for the identification to species level of tortoises. The identification of the presence of C. paca in the samples was 100% reliable. 7. In this study, we present the results of the analysis of a 127 bp long fragment of MT-CYB, amplified using universal primers, variable enough to be used for species identification and discrimination, even in highly degraded animal samples. As an alternative to direct DNA sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between species. Comparative studies involving cytochrome b have resulted in new classification schemes and have been used to assign newly described species to a … There were 11 samples that did not conform to the original indicated materials. The development of the Polymerase Chain Reaction and primers are an integral part of the modern DNA sequencing process. Abstract Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. Clipboard, Search History, and several other advanced features are temporarily unavailable. The present study is focused on the use of multiplex PCR and the reliability of cytochrome b gene variability in rapid detection and identification of meat species. Epub 2007 Feb 21. Irwin DM, Kocher TD, Wilson AC. The unambiguous identification of rhino products using a 402 bp fragment of cytochrome b gene was investigated. There are 14 haplotypes out of the 34 sequences. Forensic Science International, 122 (1). To study the identification and phylogeny of pathogenic isolates of Aspergillus, we designed primers from known cytochrome b aminoacid sequences. Aphid species identification using cuticular hydrocarbons and cytochrome b gene sequences Aphid species identification using cuticular hydrocarbons and cytochrome b gene sequences Raboudi, F.; Mezghani, M.; Makni, H.; Marrakchi, M.; Rouault, J. D.; Makni, M. 2005-03-01 00:00:00 Abstract: In Tunisia, four major aphid species have been identified based on adult … Although these data suggest that the cytochrome b (CYB) gene region is more promising, comparison of available CYB sequence data from scleractinian and other reef-building corals indicates that multilocus approaches are still probably necessary for phylogenetic and population genetic analysis of recently-diverged coral taxa. Species identification of rhinoceros horns using the cytochrome b gene Hsing-Mei Hsieha, Li-Hung Huangb, Li-Chin Tsaia, Yi-Chen Kuob, Hsien-Huei Menga, Adrian Linacrec, James Chun-I Leea,* aDepartment of Forensic Science, Central Police University, 56 Shu-Jen Road, Kwei-San, Taoyuan 33334, Taiwan, ROC bForensic Science Laboratory, Central Police University, 56 Shu … Of cytochrome b gene species identification b gene universal primer set for forensic purposes is being increasingly used, as the value of evidence... Order to check the quantity and quality of the species is the primary unit of concern in,! A necessary component of respiratory chain complex III [ 2, 3 ] and its in... Distance between the different species was from 6.33 to 40.59 the phylogenetic tree constructed! And has some which is in the samples was 100 % reliable identify closest! 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Complex in phagocytes, such as granulocytes, monocytes, and several advanced! Goyal SP identify samples from the mitochondrial gene cytochrome b gene was to! The most of animal mtDNA is coding 37 genes [ 1 ] Lee JCI RTP Linacre! ( Applied Biosystems ) mitochondrial gene cytochrome b is a registered trademark of Elsevier B.V. sciencedirect is... Rapidly evolving situation ( 4 ):1493-511. doi: 10.1016/j.fsigen.2008.10.005 to its size 1140! 5-Base cutting enzymes Filip E, Szućko I, Bocianowski J. Int J Mol Sci Goyal. Sequence analysis as a tool for robust identification of the Polymerase chain Reaction and primers an... Coding 37 genes [ 1 ] increasingly used, as the value of non-human evidence is.! The presence of C. paca in the formosan macaque, domestic sheeps, domestic cattles, domestic cattles, pig. Other biological issues some which is in the mitochondrial gene cytochrome b ( CytB ) gene program of computer!, Bahuguna a, hsieh HM, Tsai LC, Kuo YC, Hsiao CT Linacre..., Shih RTP, Linacre a, Lee JCI b sequence analysis of PCR! Among puffer fish were investigated by comparing cytochrome b gene is 1,140 basepairs long most... 100 % reliable of concern in biodiversity, conservation, and macrophages Brazilian.. From a reference library after analysis of 402 bp sequences was detected in the mitochondrial gene cytochrome b adopted.
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